Application | Comment | Organism |
---|---|---|
synthesis | enzyme is used for the industrial production of D-amino acids | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
C172A | mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity | Agrobacterium tumefaciens |
C172S | mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity | Agrobacterium tumefaciens |
General Stability | Organism |
---|---|
stable in absence rather than in presence of 2-mercaptoethanol | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | - |
Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Agrobacterium tumefaciens |
34000 | - |
2 * 34000, calculation from nucleotide sequence | Agrobacterium tumefaciens |
67000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens NTRRL B11291 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.1 | - |
- |
Agrobacterium tumefaciens |
Storage Stability | Organism |
---|---|
4°C, phosphate buffer, progressive loss of activity | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Agrobacterium tumefaciens | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? | |
N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Agrobacterium tumefaciens NTRRL B11291 | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Agrobacterium tumefaciens |
dimer | 2 * 34000, calculation from nucleotide sequence | Agrobacterium tumefaciens |